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Addgene inc pxr004
Pxr004, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pxr004+casrx+pre+grna+cloning+backbone/pXR004%3A+CasRx+pre-gRNA+cloning+backbone+(Plasmid+%23109054)/pm41397992-208-16-17
Average 93 stars, based on 21 article reviews
pxr004 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Cloning:

Article Title: A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila
Article Snippet: .. The original plasmids we used for this project were obtained from Addgene: pCFD3 (#49410), pCFD5 (#73914), pACG:eCFP (#32597), pDsRed-attP (#51019), Ac5-Stable2-Neo (#32426), pC0056-LwaCas13a-msfGFP-NES (#105815), pC0040-LwaCas13a crRNA backbone (#103851), pC0046-EF1a-PspCas13b-NES-HIV (#103862), pC0043-PspCas13b crRNA backbone (#103854), pC0054-CMV-dPspCas13b-longlinker-ADAR2DD (E488Q/T375G) (103870), pXR001: EF1-CasRX-2A-eGFP (#109049), pXR004: CasRX pre-gRNA cloning backbone (#109054), pBID-UASc (#35200), [ , , , , , , – ]. ..

Article Title: A virus-induced circular RNA maintains latent infection of Kaposi sarcoma herpesvirus
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209 ; http://n2t.net/addgene:153209 ; RRID:Addgene_153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; http://n2t.net/addgene:109054 ; RRID:Addgene_109054). ..

Article Title: TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.
Article Snippet: .. CARPIDBASU-dCasRxwasa gift from JianYan&LiangZhang (Addgene plasmid # 153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054). ..

Article Title: A virus-induced circular RNA maintains latent infection of Kaposi’s sarcoma herpesvirus
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209 ; http://n2t.net/addgene:153209 ; RRID:Addgene_153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; http://n2t.net/addgene:109054 ; RRID:Addgene_109054). ..

Article Title: Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA Intrusion
Article Snippet: The cells were transfected with jetPRIME transfection reagent (Polyplus, 101000046) or Lipomaster 3000 (Vazyme, TL301) according to the manufacturer's protocol. .. The plasmids used for transfection included pcDNA3.1-neo (our laboratory), pXR004:CasRX pre-gRNA cloning backbone (Addgene, 109054), pcDNA3.1(+) EGFP (Addgene, 129020), pGL4.23[luc2-minP] (Addgene, 226458), pGL4.74[hRluc/TK] (Promega, E6921), and pUC19 (Addgene, 50005). ..

Article Title: Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA Intrusion
Article Snippet: The cells were transfected with jetPRIME transfection reagent (Polyplus, 101000046) or Lipomaster 3000 (Vazyme, TL301) according to the manufacturer’s protocol. .. The plasmids used for transfection included pcDNA3.1-neo (our laboratory), pXR004:CasRX pre-gRNA cloning backbone (Addgene, 109054), pcDNA3.1(+) EGFP (Addgene, 129020), pGL4.23[luc2-minP] (Addgene, 226458), pGL4.74[hRluc/TK] (Promega, E6921), and pUC19 (Addgene, 50005). ..

Article Title: A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila
Article Snippet: .. The original plasmids we used for this project were obtained from Addgene: pCFD3 (#49410), pCFD5 (#73914), pACG:eCFP (#32597), pDsRed-attP (#51019), Ac5-Stable2-Neo (#32426), pC0056-LwaCas13a-msfGFP-NES (#105815), pC0040-LwaCas13a crRNA backbone (#103851), pC0046-EF1a-PspCas13b-NES-HIV (#103862), pC0043-PspCas13b crRNA backbone (#103854), pC0054-CMV-dPspCas13b-longlinker-ADAR2DD (E488Q/T375G) (103870), pXR001: EF1-CasRX-2A-eGFP (#109049), pXR004: CasRX pre-gRNA cloning backbone (#109054), pBID-UASc (#35200), [ , , , , , , – ]. ..

Article Title: TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054). ..

Plasmid Preparation:

Article Title: A virus-induced circular RNA maintains latent infection of Kaposi sarcoma herpesvirus
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209 ; http://n2t.net/addgene:153209 ; RRID:Addgene_153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; http://n2t.net/addgene:109054 ; RRID:Addgene_109054). ..

Article Title: TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.
Article Snippet: .. CARPIDBASU-dCasRxwasa gift from JianYan&LiangZhang (Addgene plasmid # 153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054). ..

Article Title: A virus-induced circular RNA maintains latent infection of Kaposi’s sarcoma herpesvirus
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209 ; http://n2t.net/addgene:153209 ; RRID:Addgene_153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; http://n2t.net/addgene:109054 ; RRID:Addgene_109054). ..

Article Title: TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation
Article Snippet: .. CARPID BASU-dCasRx was a gift from Jian Yan & Liang Zhang (Addgene plasmid # 153209). pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054). ..

Transfection:

Article Title: Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA Intrusion
Article Snippet: The cells were transfected with jetPRIME transfection reagent (Polyplus, 101000046) or Lipomaster 3000 (Vazyme, TL301) according to the manufacturer's protocol. .. The plasmids used for transfection included pcDNA3.1-neo (our laboratory), pXR004:CasRX pre-gRNA cloning backbone (Addgene, 109054), pcDNA3.1(+) EGFP (Addgene, 129020), pGL4.23[luc2-minP] (Addgene, 226458), pGL4.74[hRluc/TK] (Promega, E6921), and pUC19 (Addgene, 50005). ..

Article Title: Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA Intrusion
Article Snippet: The cells were transfected with jetPRIME transfection reagent (Polyplus, 101000046) or Lipomaster 3000 (Vazyme, TL301) according to the manufacturer’s protocol. .. The plasmids used for transfection included pcDNA3.1-neo (our laboratory), pXR004:CasRX pre-gRNA cloning backbone (Addgene, 109054), pcDNA3.1(+) EGFP (Addgene, 129020), pGL4.23[luc2-minP] (Addgene, 226458), pGL4.74[hRluc/TK] (Promega, E6921), and pUC19 (Addgene, 50005). ..



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Qualitative validation of transfection efficiency by GFP visualization. Transfection efficiency and GFP expression in Caco-2 cells inspected by inverted fluorescence microscope at 10x objective magnification at 48 hours post-transfection. (A): GFP was observed in cells cultured in the plates that were transfected with <t>CasRX</t> pregRNA expression vector and pcDNA3_EF1a-CasRx-2A-EGFP vector, and (B): shows the same cells with normal visible light.
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Image Search Results


Qualitative validation of transfection efficiency by GFP visualization. Transfection efficiency and GFP expression in Caco-2 cells inspected by inverted fluorescence microscope at 10x objective magnification at 48 hours post-transfection. (A): GFP was observed in cells cultured in the plates that were transfected with CasRX pregRNA expression vector and pcDNA3_EF1a-CasRx-2A-EGFP vector, and (B): shows the same cells with normal visible light.

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Evidence for a TMAO-HULC-miRNA regulatory axis in colorectal cancer cells

doi: 10.22088/IJMCM.BUMS.14.3.900

Figure Lengend Snippet: Qualitative validation of transfection efficiency by GFP visualization. Transfection efficiency and GFP expression in Caco-2 cells inspected by inverted fluorescence microscope at 10x objective magnification at 48 hours post-transfection. (A): GFP was observed in cells cultured in the plates that were transfected with CasRX pregRNA expression vector and pcDNA3_EF1a-CasRx-2A-EGFP vector, and (B): shows the same cells with normal visible light.

Article Snippet: The sgRNA was cloned CasRX pre-gRNA cloning backbone (Addgene 109054) that was linearized with BbsI restriction enzyme(36).

Techniques: Biomarker Discovery, Transfection, Expressing, Fluorescence, Microscopy, Cell Culture, Plasmid Preparation